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Top: Raw microscope images from channels IA, ID, and IF for cells expressing linked construct mAmetrine-tdTomato plus excess mAmetrine. Bottom: Color coded cells after analysis for cells expressing linked construct mAmetrine-tdTomato plus excess mAmetrine giving the ratio of acceptor in construct (fA, expected value = 1), ratio of donor in construct (fD, expected value < 1), and absolute concentration ratio (R, expected value < 1). Scale bar insert: 55 μm
