Table 2.
Average FRET stoichiometry values for cell expression of combinations of linked and unlinked molecules. The results determined using the generalized (G) stoichiometry theory and the original stoichiometry theory are compared to illustrate points of differentiation. The expected values for the given transfection type are given in parentheses. The number of individual cells used to calculate the average and standard deviation (SD) for each transfection type are noted in row 2.
| linked construct | free donor and acceptor | linked construct + acceptor | linked construct + donor | |
|---|---|---|---|---|
|
| ||||
| Number of cells | 59 | 42 | 17 | 18 |
|
| ||||
| fD ± SD(G) | 1.0 ± 0.2(1) | 0.0 ± 0.1(0) | 0.8 ± 0.3(1) | 0.2 ± 0.1 |
| fA ± SD(G) | 1.0 ± 0.3(1) | 0.1 ± 0.1(0) | 0.2 ± 0.1 | 0.8 ± 0.3(1) |
| fD ± SD | 1.0 ± 0.2(1) | −0.2 ± 0.1(0) | 0.5 ± 0.3(1) | 0.0 ± 0.1 |
| fA ± SD | 1.0 ± 0.2(1) | −0.3 ± 0.2(0) | 0.2 ± 0.1 | 0.0 ± 0.3(1) |