Fig. 4.

Analytical calculation of the eigenvalues of the M matrix for different cone angles α and β. Note that these parameters do not change as a function of mean orientation, $\left\{{\text{Φ}}_0,{\text{Θ}}_0\right\}$. Furthermore, they are not affected by experimental variables such as defocus, emission wavelength, or microscope NA. (a)-(c) Eigenvalue calculations, β = α, β = α/2, and β = α/8 respectively.