Fig. 5.

The image of any single molecule, fixed or rotationally mobile, may be decomposed into a linear combination of six basis functions. These six basis functions have been calculated at three representative defocus depths, given the simulation parameters presented in the main text. The x/y polarized components of these basis functions are also shown with x/y superscripts. Units of intensity are scaled such that the brightest pixel for a dipole parallel to the focal plane at 0.55 μm defocus has a magnitude of 1.