. 2015 Apr 10;59(5):2807–2816. doi: 10.1128/AAC.00056-15

TABLE 4.

Kanamycin MICs for Kmr and other A1408 methyltransferase mutants

Proposed function	Kmr		KamB^a		NpmA^b
Proposed function	Substitution	MIC (μg/ml)	Substitution	MIC (μg/ml)	Substitution	MIC (μg/ml)
SAM binding	D30A	20–50	D30A	20	D30A	8
	D55A	1,000	D55A	10	D55A	512
	T192A	>1,000	T191A	10	S195A	1,024
16S rRNA binding			R195A	400	K199A	1,024
	H197A	>1,000	R196A	10	R200A	1,024
			R201A	10	R205A	1,024
	R204A	>1,000	R203A	800	R207A	16^c
	R205A	>1,000
A1408 positioning/catalysis	W107F	1,000	W105F	10
	W107A	10	W105A	10	W107A	8
	W107A/F144A	20
	F144A	>1,000
	F144A/W194A	20–50
	W194A	400–800	W193A	10	W197A	8
	W194F	800	W193F	10

Data for KamB are from reference 25.

Data for NpmA are from reference 28.

MIC for NpmA-R207A is from this study.