TABLE 1.

Bacterial study strains, antibiotic susceptibilities, and genetic mutations in select loci

Strain,a drug exposure	MIC ofb:				Mutation(s)c in:
	DAP	VAN	OXA	CLR	mprF	rpoB	rpoC	dltA	cls2	yycF	yycG
Clinical isolates
J01	0.5	2	32	256	—	—	—	—	—	—	—
J03	3	2	16	256	T345X	S464P	—	—	—	—	—
In vitro strains
DAP
D1	32	2	0.01	16	L826F	—	N735K	—	R320L	—	—
D2	32	2	0.01	32	L826I	—	N735K	—	R320S	—	369delQ
D3	12	2	0.01	32	L826I	—	N735K	—	R320S	—	369delQ
D4	8	2	0.01	8	L826F	—	N735K	—	—	—	—
D5	24	1.5	0.01	32	P314L	—	—	—	F85X	—	—
DAP+CLR
DC1	3	2	0.01	64	H376Y, W424C	—	—	—	—	—	—
DC2	1.5	1.5	0.01	64	A302V	—	—	—	—	—	—
DC3	8	3	0.01	32	P314L	—	—	—	—	—	—
DC4	3	1.5	0.02	32	M347R	—	—	—	—	—	—
DC5	3	2	0.01	32	S337L	—	—	—	—	—	—
DAP+LZD
DL1	32	2	0.01	32	S337L	—	—	—	F85X	—	—
DL2	64	4	0.01	0.03	41insN	—	—	—	—	—	—
DL3	192	1.5	0.01	0.01	L826F	—	—	—	L77F	—	G199E
DL4	64	3	0.03	1	S295L	—	—	—	—	—	—
DL5	192	2	0.01	0.01	L826F	—	—	—	L77F	—	G199E
DAP+OXA
DO1	0.02	1.5	256	2	—	—	N735K	A426E	—	—	—
DO2	1	3	64	4	—	—	N735K	—	—	—	—
DO3	3	3	128	4	—	—	N735K	—	—	—	—
DO4	0.05	2	128	4	—	—	N735K	—	—	—	—
DO5	3	3	128	0.06	—	S366F	—	—	—	—	—
DAP+SXT
DS1	24	2	4	32	L826I	—	N735K	—	—	—	—
DS2	96	2	4	32	L826I	—	N735K	—	—	—	—
DS3	32	3	0.5	16	L826I	—	N735K	—	R320L	—	—
DS4	12	3	32	8	S295L	—	N735K	—	—	K151N	—
DS5	64	2	16	8	L826I	V1130K, V1162I	—	—	A56G, T58N	—	—

^aAll in vitro strains were derived from J01 following 28 days of antibiotic exposure.

^bMICs to antibiotics are provided in mg/liter. Susceptibility breakpoints for S. aureus are as follows: DAP, ≤1 mg/liter; VAN, ≤2 mg/liter; OXA, ≤2 mg/liter; CLR, ≤2 mg/liter (17).

^c—, the strain has the wild-type sequence.