TABLE 2.
Antimicrobial resistance profiles and genetic characteristics of K. pneumoniae isolates from animal infection sitesa
| Strain | Source | Hospitalization required? (reason) | Resistance phenotype | ST | ESBL/pAmpC gene(s)c | Other antibiotic resistance genes and integronsd | Amino acid substitution in: |
|
|---|---|---|---|---|---|---|---|---|
| GyrA | ParC | |||||||
| KM907/06 | Dog urine | Yes (esophagus perforation) | AmpC CIP CHL NAL SMX | 11 | blaDHA-1 | aadA2-like, qnrB, aac(6′)-Ib-cr, catA1, catB3-like, sul1, arr1, intI1 | Ser83Ile | Ser80Ile |
| KM60/13 | Dog genital swab | No (OPP) | AmpC CIP CHL NAL SMX KAN | 11 | blaDHA-1 | aph(3′)-Ia, qnrB, aac(6′)-Ib-cr, catB3-like, sul1, mrx-mphA, arr1, intI1 | Ser83Ile | Ser80Ile |
| KM1350/12 | Dog pus | Yes (cruciate ligament rupture) | Same as for KM60/13 | 11 | blaDHA-1 | Same as for KM60/13 | Ser83Ile | Ser80Ile |
| IMD842/11 | Dog urine | Yes (stomach ulcer) | AmpC CIP CHL NAL TMP SMX KAN | 11 | blaDHA-1 | aph(3′)-Ia, aadA2-like, qnrB, aac(6′)-Ib-cr, catA1, catB3-like, dfrA12, sul1, mrx-mphA, arr1, intI1 | Ser83Ile | Ser80Ile |
| KM256/11 | Dog pus | No (OPP; wound care after accident) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM2/11 | Dog blood culture | Yes (intoxication) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM141/10 | Dog BAL fluid | Yes (pneumonia) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1051/12 | Cat urine | Yes (urolithiasis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM900/11 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM917/11 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1152/11 | Cat urine | Yes (feline lower urinary tract disease) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1170/11 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1312/11 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1140/12 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1209/11 | Dog urine | OPP (immunosuppression) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM524/10 | Dog pus | Yes (hit by car) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1096/12 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM987/12 | Dog urine | No (OPP; protein-losing enteropathy) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM1126/12 | Dog blood culture | Yes (leptospirosis) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM686/10 | Cat urine | No (OPP; fibrosarcoma) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| 210237006 | Dog urine | Yes (hit by car) | Same as for IMD842/11 | 11 | blaDHA-1 | Same as for IMD842/11 | Ser83Ile | Ser80Ile |
| KM899/09b | Dog urine | Yes (leptospirosis) | 3GCs CIP CHL NAL TMP SMX KAN STR | 1463 | blaCTX-M-1 | aph(3′)-Ia, aadA2-like, dfrA12, strB, sul1, sul2, mrx-mphA | Ser83Ile | Ser80Ile |
| KM749/09 | Dog urine | No (OPP; UTI) | AmpC, CIP CHL NAL TMP SMX KAN STR | 1463 | blaCMY-2, blaCTX-M-1 | aph(3′)-Ia, aadA2-like, dfrA12, strB, su11 sul2 | Ser83Ile | Ser80Ile |
| KM65/09 | Dog urine | No (OPP; UTI) | AmpC CIP CHL NAL TMP SMX KAN STR TET | 1463 | blaCMY-2, blaCTX-M-1 | aph(3′)-Ia, aadA2-like, dfrA12, strB, sul1, sul2, tet(A) | Ser83Ile | Ser80Ile |
| KM822/09b | Dog urine | Yes (leptospirosis) | Same as for KM899/09 | 1463 | blaCMY-2, blaCTX-M-1 | Same as for KM899/09 | Ser83Ile | Ser80Ile |
OPP, outpatient pet; UTI, urinary tract infection; ST, sequence type; 3GCs, 3rd-generation cephalosporins; AmpC, 3rd-generation cephalosporins and beta-lactamase inhibitors; CIP, ciprofloxacin; CHL, chloramphenicol; NAL, nalidixic acid; TMP, trimethoprim; SMX, sulfamethoxazole; KAN, kanamycin; STR, streptomycin; TET, tetracycline.
KM899/09 and KM822/09 were isolated from the same animal.
All strains also had the blaOXA-1 gene.
Genes and functions: aadA, streptomycin adenylyltransferase; aph(3′)-Ia, kanamycin O-phosphotransferase; qnrB, quinolone resistance protein; aac6′-Ib-cr, variant of aminoglycoside N(6′)-acetyltransferase–ciprofloxacin-modifying enzyme; cat, chloramphenicol acetyltransferase; dfrA12, dihydrofolate reductase for trimethoprim resistance; sul, dihydropteroate synthetase for sulfonamide resistance; mrx-mphA, macrolide inactivation resistance protein-phosphotransferase; arr1, rifampin ADP ribosyltransferase; tet, tetracycline efflux; str, streptomycin phosphotransferase; intI1, integrase.