TABLE 3.
Characteristics of 3rd-generation-cephalosporin-resistant E. coli recipients after conjugation with K. pneumoniae strains or electrotransformation with K. pneumoniae plasmid DNAa
| K. pneumoniae donor | Origin | E. coli transformant | Transformation | Resistance phenotype | Carbapenemase gene | ESBL/pAmpC gene | Other bla gene(s) | Other antibiotic resistance genes and integronsb | Replicon | Inc group |
|---|---|---|---|---|---|---|---|---|---|---|
| 5012.10 | Human | NW5A | C | 3GCs STR SMX | blaCTX-M-3 | blaTEM-1 | sul2, strB | FII | IncFII | |
| 5109.57 | Human | NW8C | C | 3GCs | blaCTX-M-14 | FII | IncFII | |||
| KM749/09 | Dog | NW10A | C | AmpC STR | blaCMY-2 | I1 | IncI1-alpha | |||
| NW10F | CI, E | 3GCs CHL TMP STR SMX KAN | blaCTX-M-1 | aph(3′)-Ia, aadA2-like, dfrA12, sul1 | R | not assigned | ||||
| KM256/11 | Dog | NW11C | NC, E | AmpC STR SMX KAN | blaDHA-1 | blaOXA-1 | aph(3′)-Ia, qnrB, aac6′-Ib-cr, catB3-like, mrx-mphA, arr-1, intI1 | R | Not assigned | |
| KM899/09 | Dog | NW12A | NC, E | 3GCs TMP STR SMX KAN | blaCTX-M-1 | aph(3′)-Ia, aadA2-like, dfrA12, sul2 | R | Not assigned | ||
| 4906.28 | Human | NW15C | C | 3GCs GEN TMP STR TET SMX | blaCTX-M-15 | blaOXA-1 | aac(3)-IIc, aadA1-like, strB, qnrB, aac(6′)-Ib-cr, dfrA14, sul2, tet(A), intI1 | FIIk | IncF | |
| NW4906 | C | 3GCs | blaOXA-48 | L/M | IncL/M | |||||
| 5010.55 | Human | NW18A | C | 3GCs SMX | blaCTX-M-1 | sul2 | X1 | IncX1 | ||
| 5203.77 | Human | NW5203 | C | 3GCs TET TMP KAN SMX | blaCTX-M-15 | blaOXA-1, blaTEM-1 | aac(6′)-Ib-cr, dfrA12, sul1, mrx-mphA, tet(A), intI1 | FIIk | IncF |
C, conjugative; CI, conjugative only together with the blaCMY-2 plasmid (IncI1); NC, nonconjugative; E, plasmid transformation by electroporation; Inc, incompatibility group; ST, sequence type; 3GCs, 3rd-generation cephalosporins; AmpC, 3rd-generation cephalosporins and beta-lactamase inhibitors; CHL, chloramphenicol; TMP, trimethoprim; SMX, sulfamethoxazole; KAN, kanamycin; GEN, gentamicin; STR, streptomycin; TET, tetracycline.
Genes and functions: aadA, streptomycin adenylyltransferase; aac(3)-IIc, gentamicin acetyltransferase; aph(3′)-Ia, kanamycin O-phosphotransferase; qnrB, quinolone resistance protein; aac6′-Ib-cr, variant of aminoglycoside N(6′)-acetyltransferase–ciprofloxacin-modifying enzyme; cat, chloramphenicol acetyltransferase; dfr, dihydrofolate reductase for trimethoprim resistance; sul, dihydropteroate synthetase for sulfonamide resistance; mrx-mphA, macrolide inactivation resistance protein-phosphotransferase; arr-1, rifampin ADP ribosyltransferase; tet(A), tetracycline efflux; strB, streptomycin phosphotransferase; intI1, integrase.