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. 2015 Apr 10;59(5):2713–2719. doi: 10.1128/AAC.04908-14

FIG 4.

FIG 4

Complementation of the ΔcyaA mutant. (A) Persister levels of the ΔcyaA mutant, the ΔcyaA mutant with pUA66 (empty vector), and the ΔcyaA mutant with pUA66-cyaA were monitored for 5 h during OFL treatment. (B) Persisters were treated with GENT (10 μg/ml) and carbon sources (normalized to 60 mM carbon) in M9 minimal medium for 2 h. The persister survival fraction for each data point was normalized to that for the no-carbon-source (DI H2O; double-distilled water [ddH2O] in the figure) control. *, statistically significant difference (P ≤ 0.05) by comparison of each carbon source-treated sample and the DI H2O control for each strain. (C to E) Survival fractions of E. coli MG1655 ΔcyaA (C), ΔcyaA with pUA66 (D), and ΔcyaA with pUA66-cyaA (E) after being treated with GENT and carbon sources (black bars) or GENT, carbon sources, and 5 mM KCN (gray bars).