FIG 5.

Role of TAK1 in GRA-mediated antileishmanial activity. (A) RAW 264.7 cells were infected with L. donovani and treated with 20 μM GRA for various time periods, and the levels of total and phosphorylated TAK1 were determined by Western blotting. (B and C) Cells were transiently transfected with WT or DN TAK1 expression plasmid (24 h) and then infected with L. donovani and treated with GRA (4 h). The levels of total and phosphorylated p38 (B) and MKK3/6 (C) were determined by Western blotting. (D) WT or DN TAK1-transfected cells were treated with SB203580 for 1 h before infection, and GRA stimulation and total and phosphorylated p38 levels were determined by Western blotting. The bands were analyzed densitometrically, and fold changes are indicated below each blot. All experiments were repeated at least three times each and one set of representative data is shown.