Table 2.

Summary of the main findings from this study investigating the effects of alcohol and morphine administration and the current study.

	Morphine+Alcohol LORR	Iba1 count	Iba1 Morphology	NF-κB count	NF-κb Intensity
Wild-type	++++	M+A ++++	M+A Activated	M+A ++++	M+A ++
Tlr2−/−	−−−	−−−	M+A Resting	−−−	−−−
Tlr4−/−	++++++	−−−	M+A Activated	−−−	−−−*
Myd88−/−	−−−
IL-1ra	++++
(+)-Naloxone	++++
(−)-Naloxone	−−−
NF-κB-NBD	++

LORR = Loss of righting reflex. M = morphine, A= Alcohol. Number of plus signs signifies approximate increase in the magnitude of response, whilst the minus signs signify blockade of the response.

^*Elevated levels of staining intensity basally compared to other groups, but no effect of drug treatment.