FIG. 8.

Quantification of total collagen content. (A) Passage 2 cells, either NT (black bars) or expressing p21 shRNA (p21sh, gray bars), were formed into chondrogenic pellet cultures that were harvested at days 9, 18, and 28. Papain-digested samples were analyzed for total collagen content by hydroxyproline assay. Data are presented as total collagen per pellet (left) or total collagen normalized to DNA content (right), combined from n≥5 total from two independent cell preparations. Groups not sharing a letter indicate p<0.05. (B) Pellets from passage 5 cells harvested and analyzed as in (A).