FIG. 9.

Immunohistochemistry for type II collagen. (A) Passage 2 cells, either NT or expressing p21 shRNA (p21sh), were formed into chondrogenic pellet cultures that were harvested at days 9, 18, and 28. Pellets were cryosectioned and labeled with primary antibody to type II collagen (red) and hematoxylin (nucleus, blue). Images are representative of pellets from two independent cell preparations. (B) Pellets from passage 5 cells cryosectioned and labeled as in (A). Color images available online at www.liebertpub.com/tea