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. 2015 Mar 6;21(7-8):1455–1470. doi: 10.1089/ten.tea.2014.0363

FIG. 4.

FIG. 4.

Elastin protein released into pooled media (A) or deposited within cell matrix (B), sulfated GAGs released into pooled media (C) or deposited within cell matrix (D), and HA released into pooled media (E) or deposited within cell matrix (F), when GSNO (0–100 nM) was supplemented to HA-SMC cultures, in the presence or absence of EC cocultures. Matrix elastin and tropoelastin were quantified using a Fastin® assay, sGAGs in pooled media and matrix quantified using a quantitative dye-biding sGAG Assay, and HA amounts in pooled media and matrix quantified using a hyaluronan enzyme-linked immunosorbent quantitative assay. Data shown represent mean±standard error of protein synthesis after 21 days of culture, normalized to cell count within respective cases (n=6/condition). *p<0.05 compared with GSNO-free cultures; #p<0.01 for no cocultures versus EC coculture, at a given GSNO dosage.