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. 2015 Mar 19;21(7-8):1388–1397. doi: 10.1089/ten.tea.2014.0280

FIG. 7.

FIG. 7.

Biochemical and gene expression assays of cultured tissues. The content and variation of collagen II (A), GAG (B), and DNA (C) were measured quantitatively during the culture period as described in the Materials and Methods section. MC combined PCL-HA scaffolds had a larger amount of Collagen II, GAG, and DNA accumulation than the BMSC-seeded PCL-HA scaffolds. The gene expression levels of collagen II (D), GAG (E), and SOX9 (F) was normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and compared to the 1 week in vitro culture time point. Similarly, the expression of collagen II, GAG, and SOX9 in MC-PCL-HA scaffolds were higher than in BMSC seeded scaffolds during culture. *p<0.05, **p<0.01.