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Detailed Information of the Primer Sequences for RT-PCR
| Gene | Primer nucleotide sequence |
|---|---|
| GAPDH | Forward: 5-TCACCATCTTCCAGGAGCGA-3 |
| Reverse: 3-CACAATGCCGAAGTGGTCGT-5 | |
| Type II collagen | Forward: 5-GGCAATAGCAGGTTCACGTACA-3 |
| Reverse: 3-TTCACCCCGTTCTGACAATAGC-5 | |
| Sox9 | Forward: 5-CACACAGCTCACTCGACCTTG-3 |
| Reverse: 3-GCTCTACTAGGATTTTATTGGCTT-5 | |
| Aggrecan | Forward: 5-TCGAGGACAGCGAGGCC-3 |
| Reverse: 3-AGAGATGTGCGATGTGGGAGCT-5 |
