FIG. 3.
Expression of SMC-related genes. HF-MSCs and BM-MSCs cultured in the presence TGF-β1 (2 ng/mL) and ascorbic acid (300 μM) for 4 days. Total RNA was isolated, reverse transcribed to cDNA, and used for qRT-PCR to measure the levels of the indicated genes. The data were normalized to GAPDH. Each experiment was done in triplicates. (*) Denotes significance to HF-1 and (#) denotes significance to HF-1, HF-2, and BM-1. qRT-PCR, quantitative real-time PCR; SMC, smooth muscle cell; TGF-β1, transforming growth factor-β1.