FIG. 3.

Protein expression, gene expression, and calcium imaging of mRPCs grown on PCL scaffolds and glass. (a, b) The protein expression of three photoreceptor markers in cells grown on PCL scaffolds and glass. Higher protein expression of these photoreceptor markers was detected in cells grown on PCL scaffolds than those grown on glass. (c, d) The gene expression of three photoreceptor markers in cells grown on PCL scaffolds and glass. Compared to those grown on glass, higher gene expression of these photoreceptor markers was detected in cells grown on PCL scaffolds. (e, f) Comparison of the gene expression of three photoreceptor markers between cells grown on glass and polystyrene. (g-i) Fura-2 values were analyzed between mRPCs grown on PCL scaffolds and glass. (g) Baseline Ca²⁺ levels. (h) Time course of mRPCs stimulated with 1 mM glutamate. (i) Peak response amplitudes to 1 mM glutamate. *p<0.05, **p<0.001.