Skip to main content
. 2015 Jan 26;11(6):4101–4108. doi: 10.3892/mmr.2015.3257

Figure 3.

Figure 3

Effects of lentivirus-mediated RNA interference on YAP expression and cell proliferation. (A) Fluorescence photomicrographs of 97H cells demonstrate the transduction efficiency of shControl and shYAP. Images were captured at 72 h following transfection with identical cell densities in six-well plates. Cell counts in shYAP wells were lower compared with those of the shControl wells. (B) Effects of knockdown of YAP mRNA on 97H cells, as determined by reverse transcription polymerase chain reaction. Relative quantification of mRNA was accomplished by the 2−ΔΔCt method. (C) Cell proliferation rate was measured using an MTS assay. Results are presented as the mean ± standard deviation. Proliferation rates are expressed relative to that of day one. (D) Effects of YAP knockdown on the cell cycle progression as detected by flow cytometric analysis. YAP, yes-associated protein; 97H, MHCC97H; shControl, control short hairpin RNA-transfected cells; shYAP, YAP short hairpin RNA-transfected cells.