Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Biomed Nanotechnol. 2015 Mar 1;11(3):447–456. doi: 10.1166/jbn.2015.2038

Table I.

Experimental design based on mineralization time and cell culture media.

MSCs culture media Groups/mineralization time (hours)
Group 1/0 h Group 2/12 h Group 3/24 h Group 4/96 h
DMEM (A) Culture time: 5, 10, 15 days Culture time: 5, 10, 15 days Culture time: 5, 10, 15 days Culture time: 5, 10, 15 days
OST (B) Culture time: 5, 10, 15 days Culture time: 5, 10, 15 days Culture time: 5, 10, 15 days Culture time: 5, 10, 15 days

Notes: DMEM: Dulbecco’s minimal essential media; OST: Osteogenic Media, made of DMEM supplemented with dexamethasone, ascorbic acid, and β-glycerophosphate.