Extended Data Table 1.
Genetic characteristics of CAP256-VRC26 antibodies and V1V2-directed broadly neutralizing antibodies from other donors.
Data are from the present study and from references (Walker 2009, Walker 2011, Bonsignori2011JVI). CAP256-VRC26.01-12 are derived from B cell culture, while CAP256.VRC26-I1 and –I2 (in italics) are inferred intermediates. CDRH3 lengths use Kabat notation. a, nucleotides. b, amino acids.
| a
| |||||||
|---|---|---|---|---|---|---|---|
| Donor | Antibody | VH gene | VL gene | % Divergence, Nucleotide
|
|||
| from VH | from VL | from UCA-H | from UCA-H CDRH3 | ||||
| CAP256 | VRC26-I1 | IGHV3-30*18 | IGLV1-51*02 | 2% | 4% | 3% | 8% |
| VRC26-I2 | 6% | 7% | 7% | 13% | |||
| VRC26.01 | 8% | 4% | 11% | 18% | |||
| VRC26.02 | 9% | 5% | 13% | 27% | |||
| VRC26.03 | 9% | 7% | 13% | 28% | |||
| VRC26.04 | 9% | 8% | 13% | 28% | |||
| VRC26.05 | 10% | 5% | 13% | 24% | |||
| VRC26.06 | 11% | 7% | 16% | 30% | |||
| VRC26.07 | 12% | 8% | 14% | 26% | |||
| VRC26.08 | 12% | 10% | 17% | 33% | |||
| VRC26.09 | 14% | 10% | 19% | 34% | |||
| VRC26.10 | 12% | 4% | 15% | 26% | |||
| VRC26.11 | 12% | 14% | 18% | 32% | |||
| VRC26.12 | 15% | 8% | 18% | 27% | |||
|
| |||||||
| IAVI24 | PG9 | IGHV3-33*05 | IGLV2-14*01 | 12% | 8% | ||
| PG16 | 15% | 12% | |||||
|
| |||||||
| CH0219 | CH01 | IGHV3-20*01 | IGKV3-20*01 | 16% | 11% | ||
| CH02 | 15% | 14% | |||||
| CH03 | 14% | 11% | |||||
| CH04 | 14% | 11% | |||||
|
| |||||||
| IAVI84 | PGT141 | IGHV1-8*01 | IGKV2-28*01 | 16% | 13% | ||
| PGT142 | 16% | 13% | |||||
| PGT143 | 16% | 13% | |||||
| PGT144 | 17% | 12% | |||||
| PGT145 | 17% | 17% | |||||
| b
| ||||||||
|---|---|---|---|---|---|---|---|---|
| Donor | Antibody | VH gene | VL gene | CDRH3 Length | % Divergence, Amino Acid
|
|||
| from VH | from VL | from UCA-H | from UCA-H CDRH3 | |||||
| CAP256 | VRC26-I1 | IGHV3-30*18 | IGLV1-51*02 | 35 | 6% | 5% | 8% | 20% |
| VRC26-I2 | 35 | 11% | 7% | 13% | 29% | |||
| VRC26.01 | 35 | 16% | 7% | 18% | 40% | |||
| VRC26.02 | 35 | 16% | 7% | 27% | 46% | |||
| VRC26.03 | 35 | 14% | 9% | 28% | 46% | |||
| VRC26.04 | 35 | 14% | 8% | 28% | 46% | |||
| VRC26.05 | 35 | 20% | 8% | 24% | 40% | |||
| VRC26.06 | 36 | 18% | 9% | 30% | 44% | |||
| VRC26.07 | 35 | 18% | 9% | 26% | 46% | |||
| VRC26.08 | 37 | 16% | 9% | 33% | 51% | |||
| VRC26.09 | 37 | 21% | 7% | 34% | 54% | |||
| VRC26.10 | 35 | 17% | 5% | 26% | 46% | |||
| VRC26.11 | 35 | 23% | 23% | 32% | 49% | |||
| VRC26.12 | 35 | 22% | 14% | 27% | 43% | |||
|
| ||||||||
| IAVI24 | PG9 | IGHV3-33*05 | IGLV2-14*01 | 28 | 20% | 15% | ||
| PG16 | 28 | 21% | 21% | |||||
|
| ||||||||
| CH0219 | CH01 | IGHV3-20*01 | IGKV3-20*01 | 24 | 29% | 17% | ||
| CH02 | 24 | 22% | 23% | |||||
| CH03 | 24 | 22% | 19% | |||||
| CH04 | 24 | 23% | 17% | |||||
|
| ||||||||
| IAVI84 | PGT141 | IGHV1-8*01 | IGKV2-28*01 | 32 | 28% | 21% | ||
| PGT142 | 32 | 30% | 21% | |||||
| PGT143 | 32 | 28% | 22% | |||||
| PGT144 | 32 | 31% | 23% | |||||
| PGT145 | 31 | 30% | 26% | |||||