Skip to main content
. 2015 Apr 13;10(4):e0125145. doi: 10.1371/journal.pone.0125145

Fig 7. Effects of AA and DHA on osteoclast-specific gene expression.

Fig 7

Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and Methods. Vehicle (0.08% ethanol) or LCPUFAs (40 μM) were added from day 3 in differentiating osteoclasts and from the onset of resorption (day 12–14) in mature osteoclasts. Experiments were terminated after 3 weeks. Gene expression was quantified by real time-PCR. The results are representative of three independent experiments conducted in triplicate. Diff Ocl—differentiating osteoclasts. Mat Ocl—mature osteoclasts. *P<0.05; **P<0.01; ***P<0.001 vs. vehicle. (a) P<0.05; (b) P<0.001 AA compared with DHA.