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. 2015 Apr 13;10(4):e0123721. doi: 10.1371/journal.pone.0123721

Fig 5. AMPK and Redd1 partially contribute to metformin-induced GB cell death.

Fig 5

(A) Western Blot analyses showing AMPK inhibition after specific siRNA transfection. (B) Quantification of apoptotic and necrotic cell death, using flow cytometry and Annexin-V/PI staining, 48hrs after metformin treatment and with or without AMPK inhibition. AMPK inhibition increases cell death for U87 and U251 cells and slightly inhibits metformin effect on GB cell death. (*p<0.05, **p<0.01, n = 3). (C) Western Blot analyses showing Redd1 inhibition after specific siRNA transfection. (D) Quantification of apoptotic and necrotic cell death, using flow cytometry and Annexin-V/PI staining, 48hrs after metformin treatment and with or without Redd1 inhibition. Redd1 inhibition slightly inhibits metformin effect on GB cell death (*p<0.05, **p<0.01, n = 3).