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. 2015 Apr 13;10(4):e0124768. doi: 10.1371/journal.pone.0124768

Fig 3. Analysis of RBP7 promoter, vector construct containing RBP7 promoter-eGFP, and PCR genotyping of transgenic quail.

Fig 3

A) RBP7 promoter with regulatory elements interacting with major transcription factors in adipose tissue. B) Schematic representation of the pLT-RBP7p3k-eGFP vector. Restriction sites, ClaI and PacI are depicted. LTR, long terminal repeat; ψ, packaging signal; RRE, rev-response element; cPPT, central polypurine tract of HIV-1; eGFP, enhanced green fluorescent protein; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. The arrows represent the location of forward and reverse primers used for genotyping PCR. C) Genotyping PCR of transgenic and wild-type quail using the two sets of primers depicted in the figure of B). WT, wild-type quail; +: pLT-RBP7p3k-eGFP plasmid DNA (positive control).