Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 15;26(8):1416–1427. doi: 10.1091/mbc.E14-11-1576

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Cui et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 5: — MiR-1188 mediates invasion and apoptosis by modulating Bcl-2 and Sp1 levels. (A, B) qRT-PCR analysis showed the relative levels of Bcl-2 and Sp1 in Hepa1-6 cells transfected with NC or siRNA, respectively. (C, D) Immunoblot analysis of Bcl-2 and Sp1 in Hepa1-6 cells transfected with siRNA. (E) Representative growth curves corresponding to Hepa1-6 cells transfected with NC or si-Sp1-588. (F) Migration and invasion of Hepa1-6 cells transfected with NC or si-Sp1-588. (G) Annexin V–FITC/PI staining of cells cotransfected with NC or miR-1188 inhibitor and/or si-Bcl-2 for 20 h. (H, I) Immunoblot analysis showed that the levels of Bcl-2 and Sp1 were changed in Hepa1-6 cells transfected with miR-1188 mimics, miR-1188 inhibitor, si-Bcl-2, or si-Sp1. More significantly, miR-1188 inhibitor rescued the level of Bcl-2 or Sp1 in cells cotransfected with inhibitor and siRNAs compared with cells transfected with siRNA alone. Error bars represent the SE obtained from three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001.