FIGURE 3:

Disome VIII cells exhibit DNA replication-initiation defects. (A) Plasmid loss rate was determined in wild-type (A35934, A35933), cdc6-1 (A35944, A35943), and disome VIII (A35937, A35938) cells harboring a plasmid bearing either one or eight ARS sequences as described in Materials and Methods. Graphs indicate the mean and SD of at least 12 independent cultures. Statistical tests were performed between the wild-type strain harboring the eight-ARS plasmid and the mutant or disomic strains harboring the eight-ARS plasmid, or between the wild-type strain harboring the one-ARS plasmid and the mutant or disomic strains harboring the one-ARS plasmid. *p < 0.05, **p < 0.005, ***p < 0.0005 (Wilcoxon rank-sum test). (B) Plasmid loss rate was determined in wild-type (A35934, A35933), clb5::URA3 (A36737, A36739), and disome V (A36896, A36897) cells harboring a plasmid bearing either one or eight ARS sequences as described in Materials and Methods. Graphs indicate the mean and SD of three independent cultures. Statistical tests were performed between the wild-type strain harboring the eight-ARS plasmid and the mutant or disomic strains harboring the eight-ARS plasmid, or between the wild-type strain harboring the one-ARS plasmid and the mutant or disomic strains harboring the one-ARS plasmid. *p < 0.05; **p < 0.005 (Wilcoxon rank-sum test). (C) Tenfold dilutions of euploid and disomic cells wild type or mutant for MRC1 on YPD plates, YPD plates + 50 mM hydroxyurea, YPD + HEPES, pH 7.4, or YPD + HEPES, pH 7.4, + 0.2 μg/ml phleomycin. (D) Tenfold dilutions of euploid and disomic cells wild type or mutant for TOF1 on YPD plates, YPD plates + 75 mM hydroxyurea, YPD + HEPES, pH 7.4, or YPD + HEPES, pH 7.4, + 0.2 μg/ml phleomycin.