FIGURE 2:

Deletions of FAP61 or FAP251 strongly affect ciliary motility. (A–C) Video captures of paths of swimming wild-type (A), FAP61-KO (B), and FAP251-KO (C) cells recorded for 2.5 s. (D) Average swimming distances per 2.5 s normalized to the wild-type value (n = 100 for each strain); knockout cells swam 37 ± 5.5% (FAP61-KO) and 25 ± 1.5% (FAP251-KO) of the wild-type distance. (E) Paths of swimming cells untreated or treated with 0.5 mM IBMX. (F, F′) Average distance of swimming paths normalized to either untreated wild-type cells (F) or untreated cells with the same genetic background (F′); note that IBMX treatment partially rescues the swimming defect in the mutants. (G) Growth curves of wild-type and knockout strains grown at 30°C in SPP medium with or without shaking. (H) Evaluation of the efficiency of phagocytosis based on the number of India ink–labeled food vacuoles per cell formed during 10 min (averages of three experiments). Wild type, 6.2 food vacuoles, n = 322 cells; FAP61-KO, 2.8, n = 477; and FAP251-KO, 2.1, n = 452.