FIGURE 2:

hGle1 is recruited specifically to SGs upon heat shock. (A) Endogenous hGle1 is localized to SGs. HeLa cells were either left untreated or exposed to heat shock at 45°C for 60 min. Cells were processed for immunofluorescence using anti-hGle1 and G3BP antibodies. Scale bar: 10 μm. (B) hGle1 is not a component of P bodies. Immunofluorescence of hGle1 and P-body marker Dcp1a in HeLa cells treated with either heat shock or left untreated. The white line in A and B indicates the position of the line scan to assess colocalization in merged images using ImageJ. Arrow points toward the position of white line showing colocalization. Scale bar: 10 μm.