FIGURE 4:

hGle1-dependent SG defects are not linked with microtubules. (A–C) HeLa cells transfected with CTRL and hGLE1 siRNAs were treated with (A) vehicle alone, (B) 5 μM nocodazole, or (C) 100 nM Taxol for 120 min at 37°C followed by heat shock at 45°C for 60 min. Cells were processed for immunofluorescence with anti-G3BP, α-tubulin, and hGle1 antibodies. Scale bar: 10 μm. (D) Quantification of SG numbers in hGLE1 and CTRL siRNA cells treated with indicated drugs after heat shock.