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. 2015 Apr 15;26(8):1523–1531. doi: 10.1091/mbc.E14-12-1613

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© 2015 Lu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — PKCι knockdown decreases the permeability of MDCK II monolayers. (A) PKCι was knocked down by lentiviral shRNA, and cell lysates were analyzed by Western blotting. β-Actin was used as loading control. (B) Quantification of protein levels in A. (C) TER measurement shows PKCι reduction results in a slight but significant increase of TER. (D) The 4- kDa FL-dextran flux assay shows that knockdown of PKCι significantly decreases epithelial permeability. Each experiment was performed in triplicate and repeated at least three times. Error bars represent SEM. **p < 0.001.