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. 2015 Apr 13;10(4):e0122784. doi: 10.1371/journal.pone.0122784

Fig 1. MiR-326 targets the 3′-untranslated region (UTR) of Bcl-xL but not that of Bak.

Fig 1

(a) Putative miR-326 binding sequences in the 3′-UTR of the Bcl-xL and Bak genes and the mut sequences. (b) Transfection of miR-326 mimic compared to the negative control (miR-NC) significantly inhibited luciferase activity from a reporter vector containing the 3′-UTR of Bcl-xL (**P<0.01). However, there was no inhibition of a reporter vector with mutations in the miR-326-binding site (Bcl-xL-mut) (P>0.05). (c) Transfection of miR-326 did not significantly reduce the luciferase activity from reporter vectors containing the 3′-UTR of Bak or Bak-mut (P>0.05). Results represent the means ± SD of luciferase values in 3 independent experiments and are normalized to 1.0 for the miR-NC samples.