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. 2015 Apr 13;10(4):e0122784. doi: 10.1371/journal.pone.0122784

Fig 2. MiR-326 regulates the expression of endogenous Bcl-xL.

Fig 2

(a, b) Transfection efficiency of miR-326 mimic and miR-326 inhibitor in leukocyte-depleted apheresis platelets (LDPs) were determined by qRT-PCR. LDPs were transfected using a transfection kit and mimic or inhibitor at a final concentration of 50, 100, or 200 nM and were collected after 24 h for analysis. A blank control sample and non-targeting negative RNA (miR-NC or inhibitor NC) were used as controls. *P<0.05, **P<0.01. (c) qRT-PCR of Bcl-xL mRNA was performed 24 h after transfection of LDPs as indicated. *P<0.05. (d) qRT-PCR of Bak mRNA was performed 24 h after transfection of LDPs as indicated. (e–g) Levels of Bcl-xL and Bak protein were determined by western blotting. The mean ± SD expression levels are shown. *P<0.05. Results are representative of 3 independent experiments.