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. 2015 Apr 13;10(4):e0122784. doi: 10.1371/journal.pone.0122784

Fig 4. MiR-326 promotes platelet apoptosis.

Fig 4

Leukocyte-depleted apheresis platelets (LDPs) were untransfected (control) or transfected with 50 nM of miR-326 mimic or a non-targeting negative control miRNA (miR-NC). LDPs were cultured under standard blood banking conditions and harvested at 24 or 72 h. Flow cytometry was used to analyze the apoptosis status of platelets after transfection. (a, b) Depolarization of ΔΨm was determined by JC-1 fluorochrome assay. *P<0.05. (c) Annexin V positive staining of platelets increased by nearly two-fold after transfection of miR-326 mimic. *P<0.05. (d) Overexpression of miR-326 mimic increased caspase-3 activity in LDP. *P<0.05.