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. 2015 Jan 26;19(4):734–743. doi: 10.1111/jcmm.12387

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 2 — hMSCs activate SIPS following ActD treatment. (A) Percentage of EdU positive cells on the total Hoechst 33342 positive cells at 1 and 9 days of recovery after 3 hrs of ActD treatment. Mean ± SEM of three independent experiments is shown. Student's t-test was performed and the significance value indicated by asterisks. (B) Immunoblotting of p53, p21 and p16 proteins at 0, 1, 2, 3, 6 and 9 days of recovery. β-Actin was used as loading control. Sign (-) indicates control cells. (C) Representative images of β-galactosidase staining after 21 days of recovery (phase contrast microscope 10× magnification images). (D) Immunofluorescence analysis of persistent γH2AX foci (red) after 21 days of recovery. DAPI counterstains the nuclei (blue). (E) Immunofluorescence co-localization analysis of γH2AX (green) and 53BP1 (red) after 21 days of recovery. In all panels the white bar represents 10 μm.