Fig 3.
Senescent hMSCs augment the expression of inflammatory cytokine genes and promote tumour cell migration. (A) qPCR relative gene expression levels of nine SASP factors in ActD-treated hMSCs after 9 and 15 days of recovery. Relative variation of transcript levels in ActD-treated hMSCs was expressed as Log2 (fold change), calculated by using Actβ as reference gene and time-matched control cells as calibrator. One representative experiment out of two. (B) Influence of conditioned medium obtained from senescent and control hMSCs on the migration ability of U2OS and CALU-1 cell lines. Shown are representative images (bottom) and graph (top) of migrating cell number/field (mean ± SD) derived from three independent migration assays. Student's t-test was performed and the significance values indicated by asterisks. (C) Influence of conditioned medium obtained from senescent and control hMSCs on the proliferation of U2OS and CALU-1 cell line. CellTiter-Blue fluorescence values were normalized on control cells and represented as mean ± SD of three independent experiments.