Fig 1.

High mRNA level of USP28 in NSCLC was correlated with low patients survival. After total RNA extraction, USP28 mRNA expression in normal lung tissues, MRC-5, A549, H1299, SPC-A-1, LTEP-A-2 and SK-MES-1were assayed by qRT-PCR. The USP28 expression in normal lung tissues were arbitrarily defined as 100%. Data are mean ± SD of three separate experiments (A). The mean expression of USP28 in NSCLC tissues and adjacent normal tissues were assayed by qRT-PCR. The USP28 mean expression in normal lung tissues were arbitrarily defined as 100%, Data are mean ± SD of 22 samples, N = 22 (B). The USP28 expression in tumour tissues and in adjacent normal tissues were assayed by qRT-PCR. The difference of USP28 expression between in tumour tissues and in adjacent normal tissues in each patient were compared. There are 17 of 22 cases, which showed a higher USP28 expression in tumour tissues than in adjacent normal tissues. (C). The Kaplan–Meier plot of overall survival in NSCLC patients post-operation according to the USP28 mRNA expression. The mean value of all 70 NSCLC tissues was chosen as the cut-off point for separating USP28 high expression (n = 53) from USP28 low expression cases (n = 17)(D). *P < 0.05