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. 2015 Feb 18;19(4):889–902. doi: 10.1111/jcmm.12514

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 10 — GDNF knock-down in the HK2 cell line. (A) Detection of the GDNF protein in silenced clones. GDNF immunostaining with a monoclonal anti-GDNF antibody revealed a reduction in signal between silenced (shRNA 3D, shRNA 2B) and negative control clones. The figures are representative of the results of three experiments. (B) Real-time PCR quantification of GDNF mRNA expression in silenced (white bars) and control clones (black bar). The results represent the mean ± SD of three separate experiments performed in triplicate.