Table 1.
Primer sequences
| Gene | Upstream primer | Downstream primer | Size (bp) | TM (°C) | Cycle |
|---|---|---|---|---|---|
| Quantitative comparative PCR | |||||
| GDNF | ggctatgaaacccaaggaggaactg | tgcctgccctactttgtcactc | 135 | 68 | 36 |
| Oct4 | gacaacaatgaaaatcttcaggaga | ttctggcgccggttacagaacca | 218 | 60 | 34 |
| Nestin | cagcgttggaacagaggttgg | tggcacaggtgtctcaagggtag | 346 | 60 | 32 |
| Runx-2 | cctctgacttctgcctctgg | tatggagtgctgctggtctg | 295 | 60 | 40 |
| 18S | gtaacccgttgaaccccatt | ccatccaatcggtagtagtagcg | 156 | 60 | 19 |
| G3PDH | tccaccaccctgttgctgta | accacagtccattgccatcac | 450 | 60 | 30 |
| Real-time PCR | |||||
| GDNF | ggctatgaaacccaaggaggaactg | tgcctgccctactttgtcactc | 135 | 68 | |
| Osteonectin | cctggatcttcttctccttgc | atcaggcagggcttcttgct | 71 | 60 | |
| Osteopontin | cgagacctgacatccagtacc | gatggccttgtatgcaccattc | 94 | 62 | |
| 18S | gtaacccgttgaaccccatt | ccatccaatcggtagtagtagcg | 156 | 60 | |
| G3PDH | gaaggtgaaggtcggagt | tggcaacaatatccactttacca | 92 | 62 | |