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The A27L and A2L OPT gene was enzymatically digested with HindIII and NotI. Lane 1, 1kb DNA ladder; Lane 2, 100bp DNA ladder; Lane 3, pVax1 uncut; Lane 4 pVax1 cut; Lane 5, pA27L uncut; Lane 6, pA27L cut; Lane 7, pA27LOPT uncut; Lane 8, pA27LOPT cut. The expected band of 434bp corresponding to the A27L gene is highlighted with an arrow.
