Fig 3. Effect of miR-223 on PI3K/Akt pathway.

(A) The cells were transfected with miR-223 inhibitor (miR-223 inhibitor group) or its control (control group). The cells were then sensitized with anti-2,4-dinotrophenyl (DNP) immunoglobulin E (IgE) and were triggered with DNP–human serum albumin for 10 min. Whole-cell lysates were used to examine the phosphorylation of IκB-α and Akt and their total protein levels by western blotting. (B) Nuclear extracts were used to test NF-κB by EMSA.