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. 2015 Apr 13;10(4):e0123575. doi: 10.1371/journal.pone.0123575

Fig 5. Insulin-like growth factor 1 receptor (IGF-1R) is targeted by miR-223.

Fig 5

(A and B) The cells were transfected with miR-223 mimics (mimics NC as control) or miR-223 inhibitor (inhibitor NC as control). At 24 h post-transfection, the expression of IGF-1R were determined by western blotting. (C and D) To assess whether IGF-1R is a direct target of miR-223, the cells were co-transfected with IGF-1R-3′UTR vector and miR-223 mimics (mimics NC as control) or miR-223 inhibitor (inhibitor NC as control). After 24 h of transfection, the lysates were harvested and luciferase activities were measured. Data are represented as the mean ± SD for three experiments (n = 4). *P < 0.01 compared to the mimics NC group; # P < 0.05 compared to the inhibitor NC group.