Table 5. Phylotypes of the core bacterial community in the CMC intestines.
Representative OTU | RDP classifier (Bootstrap value, %) | BLAST best hits | Percentage (%) | |||
---|---|---|---|---|---|---|
UB 1 source (Identity, %) | Known species (Identity, %) | Clone library 2 | Metagenomic 3 | Li et. al. 4 | ||
N13-23 | Bacteroides (100) | Intestinal tract of CMC (99) | Bacteroides sp. (99) | 0.8 | 7.0 | 12.2 |
N8-5 | Meniscus (100) | Intestinal tract of CMC (99) | Sunxiuqinia sp. (91) | 19.3 | 4.2 | 16.0 |
N8-1 | Marinifilum (100) | Intestinal tract of CMC (99) | Marinifilum sp. (94) | 13.8 | 0 | 10.3 |
N13-1 | Lachnospiraceae (98) | Intestinal tract of CMC (99) | Clostridium colinum (88) | 2.3 | 0 | 2.8 |
N14-3 | Clostridium XlVb (95) | Gut of Polyphagous lepidopteran larvae (91) | Clostridium colinum (90) | 5.4 | 0.2 | 0 |
N11-27 | Mollicutes 5 | Stomach of yellow catfish (97) | Candidatus Bacilloplasma (86) | 7.7 | 16.6 | 0 |
N11-5 | Mollicutes 5 | Stomach of yellow catfish (99) | Abiotrophia defectiva (84) | 11.5 | 2.1 | 0 |
N11-15 | Enterobacteriaceae (100) | Endosymbiont of Onthophagus taurus (97) | Pantoea sp. y2 (97) | 8.5 | 1.2 | 0 |
N15-67 | Vibrio (100) | Intestinal tract of CMC (99) | Vibrio aestuarianus (96) | 2.3 | 2.9 | 0.3 |
N8-2 | Alphaproteobacteria (99) | Intestinal tract of CMC (99) | Pseudovibrio denitrificans (88) | 5.4 | 0.2 | 9.7 |
De2559 | Bacteria (99) | Stomach of yellow catfish (96) | Uncultured Mycoplasmataceae (95) | 0 | 28.8 | 0 |
De3312 | Bacteroidetes (100) | Intestinal tract of CMC (100) | Dysgonomonas capnocytophagoides (87) | 0 | 6.1 | 1.3 |
1 UB, uncultured bacteria;
2 Percentage among the 128 cloned sequences (>97 identity);
3 Percentage among the 5,5713 metagenomic reads (>97 identity);
4 Percentage among 319 cloned sequences (Li et. al. (2007));
5 OTUs were classified to Firmcutes based on RDP. However, phylogenetic analysis showed they belonged to Mollicutes.