Table 1. Summary of genetic variability and sequence characteristics of the candidate barcodes and their main combinations in this study.
| rbcL | matK | trnH-psbA | ITS | ITS2 | R + M | R + M + T | R + M + I | R + M + I2 | R + M + T + I | |
|---|---|---|---|---|---|---|---|---|---|---|
| Aligned length (bp) | 614 | 718 | 857 | 680 | 241 | 1333 | 2191 | 2015 | 1575 | 2872 |
| Average intra-distance | 0.14% | 0.33% | 0.87% | 0.75% | 0.80% | 0.24% | 0.36% | 0.41% | 0.32% | 0.47% |
| Average inter-distance | 2.14% | 5.12% | 11.69% | 11.10% | 12.73% | 3.71% | 5.06% | 6.04% | 4.92% | 6.70% |
| Average theta (ө) | 0.17% | 0.24% | 0.25% | 0.48% | 0.58% | 0.21% | 0.21% | 0.29% | 0.26% | 0.29% |
| Coalescent Depth | 5.57% | 2.04% | 4.15% | 5.30% | 6.36% | 2.58% | 1.91% | 2.39% | 2.19% | 2.31% |
| Proportion of variable sites | 15.79% | 33.43% | 47.37% | 50.88% | 51.19% | 25.36% | 32.63% | 32.90% | 29.21% | 37.05% |
| Proportion of parsimony sites | 12.38% | 27.72% | 32.56% | 43.09% | 48.13% | 20.63% | 25.33% | 27.84% | 24.57% | 29.18% |
| Rate of PCR and sequencing success | 100% | 100% | 100% | 97.80% | 97.80% | N/a | N/a | N/a | N/a | N/a |
R, rbcL; M, matK; T, trnH-psbA; I, ITS; I2, ITS2.