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. 2015 Apr 13;10(4):e0124065. doi: 10.1371/journal.pone.0124065

Fig 2. F56A8.3 RNAi clone reduces the level of N. parisii infection at several stages of infection.

Fig 2

(A) Pathogen load at 8 hpi on control or F56A8.3 RNAi measured as the number of FISH-stained sporoplasms seen in intact C. elegans intestines. Data are represented as mean values with SEM from three independent, blinded experiments (**p = 0.002, paired two-tailed t-test). (B) Pathogen load at 30 hpi on control or F56A8.3 RNAi measured as the fold change in N. parisii rDNA transcript by qRT-PCR relative to L4440 infected at the lowest dose. Data are represented as mean values with SEM from three independent experiments (*p = 0.033, two-way analysis of variation, testing RNAi treatment effecting pathogen load at all doses). (C) Pathogen load at 40 hpi with C. elegans infected at the L2 stage on control or F56A8.3 RNAi measured as the average number of spores produced per animal. Data are represented as mean values with SEM from three independent experiments (***p = 0.0005, paired two-tailed t-test).