Fig 9. Effect of time axis length on aligned kymograph noise.

Kymographs with time axes varying from 20 to 200 time frames in length were aligned by both methods. The resulting column-wise variances ${\sigma }_R^2(x)$ and ${\sigma }_W^2(x)$ were calculated as in Fig 8. Here we plot, for each kymograph, the mean of these column variances, i.e., $⟨{\sigma }_R^2(x)⟩$ and $⟨{\sigma }_W^2(x)⟩$, showing that kymograph noise increases with time axis length for the Reisner method but remains constant for WPAlign.