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. 2015 Apr 13;10(4):e0122348. doi: 10.1371/journal.pone.0122348

Fig 6. Co-expression of SMN and coilin increases processing of ectopically expressed scaRNA2 in WI-38 cells.

Fig 6

(A) WI-38 cells were co-transfected with pCDNA3.1+scaRNA2 and empty GFP vector (lane 2), myc-coilin (lane 3), myc-SMN (lane 4) or myc-coilin + myc-SMN (lane 5) for 24 hrs followed by RNA isolation. RNA (10 μg/lane) was run on a 6% polyacrylamide gel, Northern blotted and detected using a DIG labeled oligo probe that detects scaRNA2 (shown in Fig 3). The positions of ectopic pre-processed full-length scaRNA2 (indicated as B), ectopic intermediate-processed scaRNA2 (denoted as A), ectopic processed full-length scaRNA2 and endogenous full-length scaRNA2 are shown. The blot was then probed for 5S ribosomal RNA (5S) to verify equivalent loading of RNA (lower panel). (B) Histogram showing quantification of the ectopic intermediate (denoted as A) to ectopic pre-processed FL (denoted as B) ratio for 3 experimental sets represented in panel A. The mobility of a DIG-labeled RNA marker (in nucleotides) is shown.Standard deviation was used to include error bars. Students t test was used to determine statistical significance, indicated by “*” and corresponding to a p value less than or equal to 0.05.