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. 2015 Apr 13;10(4):e0123009. doi: 10.1371/journal.pone.0123009

Fig 4. Pro-inflammatory and regulatory/anti-inflammatory cytokine mRNA expression correlates with low parasite load.

Fig 4

Ex-vivo analyses of relative mRNA levels for indicated genes in the splenic compartments of mongrel dogs infected with Leishmania infantum were correlated with individual log of spleen parasite load detected by pPCR for the Leishmania sp ssrRNA gene. Gene expression levels of each tested cytokine were normalized using HPRT and RP32 expression. For parasite load values, canine HPRT primers were used in order to normalize initial concentrations of DNA in each sample. P value < 0.05 indicates significant correlation, Pearson correlation test.