Fig 4. CL097 induces higher levels of PGE₂, NO₂ ^- and IFN-γ than CpG.

(A) DCs were stimulated with CpG or CL097 at concentrations of 0.2, 1, and 5 μg/ml for 2 days, and PGE₂ levels were measured from culture supernatants. Data shown are representative of two independent experiments. (B) OT-II CD4⁺ T cell/DC co-cultures were stimulated with OVA protein (25 μg/ml) in the presence or absence of 1 μg/ml CpG or CL097 for 3 days, and PGE₂ levels were measured. Data shown are representative of two independent experiments. (C) OT-II CD4⁺ T cells/DC co-cultures were stimulated in the presence or absence of TLR agonists and OVA protein (25 μg/ml) for four days, and supernatants were collected to measure NO₂ ^- by Griess assay. Data shown are representative of three independent experiments. (D) OT-II CD4⁺ T cells/DC co-cultures were stimulated in the same conditions mentioned above for 4 days, and supernatants were collected to measure IFN-γ by ELISA. Data shown are representative of three independent experiments. Statistical differences between treatments were analyzed with one-way ANOVA. The graph shows the mean ± SD of triplicate wells. *p<0.05; **p<0.005.