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M. smegmatis (Msm) strains were grown in 7H9 to saturation at 37°C and diluted 100 fold in 7H9 (pH 5.5) either in the absence (0 mM) or presence of (0.5 mM, 1 mM, or 1.5 mM) NaNO2. The growth was monitored as OD at 600 nm in Bioscreen kinetic growth reader at intervals of 3 h for 40 h. The growth was plotted using GraphPad prism 5 software.
