Fig 4. Effect of the concentration of CPAs: (A) number of cell blebs; (B) total area of cell blebs; (C) bleb index; (D) mortality rate of cells; (E) schematic of Alip and Acyto.
HeLa cells were treated with a series of solutions containing different amounts of DMSO or glycerol, as well as the fluorochromes Hoechst and PI. After 30 minutes, when cells were stable, an inverted fluorescence microscope was used to observe cell death. For (A), (B) and (C), the cell number was approximately 40. For (D), the cell number was approximately 500 and the experiment was repeated 5 times. For (E), the red boundary denotes the lipid bilayer and the green boundary denotes the cortical cytoskeleton.